Genetic transformation, regeneration and analysis of transgenic peanut

Abstract

Conventional breeding has led to the improvement of some peanut traits such as seed yield and drought tolerance. However, many important agronomic traits have yet to be improved. The recent development of transformation and regeneration systems has allowed the introduction of useful genes into peanut germplasm. The understanding of disease resistance mechanisms is becoming increasingly advanced so that it should be possible to apply technologies that have been proven in other species to peanut.In this technical report we describe protocols for the routine regeration of transformed peanut plants of spanish and virginia market types based on microprojectile bombardment of embryogenic callus as well as Agrobacterium-mediated transformation of somatic embryos.