What can we learn from population genomics studies of Curtobacterium flaccumfaciens pv. flaccumfaciens, the cause of tan spot on mungbean?

Abstract

The bacterium Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is the cause of tan spot (in some regions known as ‘wilt’) on mungbean (Vigna radiata) and other legumes worldwide. The pathogen was first reported in mungbean paddocks in Queensland in 1984, and subsequently in New South Wales in 1986, causing yield losses of up to 25% in hot and dry seasons. No chemical control is available, and disease management relies on pathogen exclusion, through the use of clean seed, and deployment of moderately susceptible varieties. Breeding programs are currently working to incorporate better resistance into new mungbean varieties. The success of breeding programs depends on a thorough understanding of the genetic diversity and evolution of the pathogen population(s). This project was designed to elucidate the genotypic diversity of Cff population(s) and investigate sources of inoculum that contribute to tan spot epidemics in the northern grains region (Queensland and northern New South Wales). Whole genome re-sequencing of 100 Cff isolates detected moderate genotypic diversity, with a narrower genetic background compared to the global Cff population. One highly adapted clonal lineage was found to dominate the population with a frequency of 45%. This study showcases how population genomics studies can be used to test hypotheses relating to disease epidemiology and provide useful information for resistance breeding programs. The results provide insights on Cff population structure and epidemiology, and have direct application for breeding programs by providing a set of Cff isolates that represent the genetic diversity of the pathogen population(s) in the northern grains region.