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Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform

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Huey, J. A., Real, K. M., Mather, P. B., Chand, V., Roberts, D. T., Espinoza, T., McDougall, A., Kind, P. K., Brooks, S. G. and Hughes, J. M. (2013) Isolation and characterization of 21 polymorphic microsatellite loci in the iconic Australian lungfish, Neoceratodus forsteri, using the Ion Torrent next-generation sequencing platform. Conservation Genetics Resources, 5 (3). pp. 737-740. ISSN 1877-7252

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Article Link: http://dx.doi.org/10.1007/s12686-013-9894-8

Abstract

We isolated and characterized 21 microsatellite loci in the vulnerable and iconic Australian lungfish, Neoceratodus forsteri. Loci were screened across eight individuals from the Burnett River and 40 individuals from the Pine River. Genetic diversity was low with between one and six alleles per locus within populations and a maximum expected heterozygosity of 0.774. These loci will now be available to assess effective population sizes and genetic structure in N. forsteri across its natural range in South East Queensland, Australia.

Item Type:Article
Business groups:Fisheries Queensland
Additional Information:Huey, Joel A. Real, Kathryn M. Mather, Peter B. Chand, Vincent Roberts, David T. Espinoza, Thomas McDougall, Andrew Kind, Peter K. Brooks, Steven Hughes, Jane M. Seqwater This microsatellite library was developed with funding assistance from Seqwater. Tinana and Burnett samples were collected under General Fisheries Permit 157 882, and AEC approvals CA2010/10/473 and CA2011/07/522. Pine River samples were collected under General Fisheries Permit No. 140615 and AEC approval CA2011/10/551. Springer Dordrecht
Keywords:Neoceratodus forsteri Lungfish Ion Torrent Microsatellite
Subjects:Aquaculture and Fisheries > Fisheries > Fishery research
Aquaculture and Fisheries > Fisheries > Fishery conservation
Live Archive:07 Oct 2014 04:47
Last Modified:28 Mar 2022 02:11

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