The preservation of bovine blood for the determination of inorganic phosphate in the diagnosis of aphosphorosis

Abstract

An improved method of preserving and processing bovine blood for the determination of the level of inorganic phosphate is described. The incomplete precipitation of protein in dilute trichloracetic acid, which is noted particularly at high atmospheric temperatures and which interferes with colorimetric estimation, may be avoided by adding fullers earth and trichloracetic acid to the filtrate prepared for treatment for colorimetric estimation. The low final concentration of trichloracetic acid attainable by this procedure permits the storage of the blood for several days without appreciable hydrolysis of organic P. compounds to inorganic P.