Barley stripe mosaic virus on Cape Barley in Queensland

Abstract

Barley stripe mosaic virus was found to occur widely in Queensland, but to be confined to Cape, a relatively unimportant fodder variety. Most of the seed stocks of this long-established variety were found to be infected at the 1-5% level. Virus was purified using charcoal, an agarose column and sucrose rate zonal centrifugation in conjunction with differential centrifugation. The top virus band in rate zonal separations was composed of three distinct but closely associated minor bands. An antiserum was produced and used in infection testing of young seedlings.