Some characteristics of Tobacco streak virus isolated from Queensland

Abstract

Tobacco streak virus (TSV) has not previously been reported from Australia. It was isolated from Dahlia pinnata, Ageratum houstonianum, Solanum auriculatum, Nicandra
physalodes, Solanum nigrum and Asclepias physocarpa, growing in scattered locations in south-eastern Queensland. Serological relationship to English and American strains of
TSV was demonstrated. Typical TSV virus particles were demonstrated by electron microscopy and a three-component system was visible in sucrose density gradient separations.
The host range and symptoms were generally similar to those of the type strain of TSV, but red node in bean (Phaseolus vu/garis) resulted from inoculation of this species with
some highly infective preparations. Antisera were produced using two different virus purification methods.