LOCUS L71 5456 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L71. ACCESSION L71 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5456) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5456) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5456 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L71" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYTTDR SDDVEPILHDADVMKFSGFVGAKDRFMLQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGTEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1616 a 1178 c 1273 g 1389 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag tttagtgtcg gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atttataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 cgattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgctccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgacc gttcaccttg 4441 taaaagcagg tgacgaggag aattatacaa cagatcgatc ggacgatgtt gaacccatac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagac aggtttatgt 4561 tacagggtgc tgatccaatc ggccctggtg acaggtctgt ccgtcgcttc aaaccgtccg 4621 ccacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggca 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaggag aagctacagg 4741 atgaagatcc aatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgata 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagtatcaag cctagattaa aagatcagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtaga tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct gggaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctacattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt ctggttccaa tccattgatc aaattggcac 5341 tgcttctggt gactacactg ccgctgaatc aacagcgtaa acaaattcct attggtcact 5401 ccgtgaaaag agagtggcta atacgcccct ttgatggcgt taaatgaaag cgtagg // LOCUS 5433 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 5433. ACCESSION 5433 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="5433" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Croppa Creek, NSW" /collection_date="2014" /collected_by="Murray Sharman" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMRKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWTNIQ AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDDQN KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNYCISYGAWTDRDMEFGMVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSIRRFRPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPVPKSVL DRLRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTNSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1624 a 1186 c 1268 g 1381 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc tactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagcttcctt ctaagaaaga taaagaagga aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttcccatgc aaccatcaac 961 cggtatgcgc tcaagttttg cgaagacttg gagcttgaca tgaccagcac tgaaatgctt 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgtcgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg taccactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagccttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgcgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcggttga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tgcctgttct tgatgctttt cacaaatgcc tatacaaagc atcagggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaagctc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 aatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccaattc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc taaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgagagattc attgtgtaca 3661 ccggtacact atcgacactc attagtgccc gacaaaactc agatagcata tcactctact 3721 caattagaga ccagagggtt agatatattg aggatgaaaa tgcgaactgg acgaacattc 3781 aagcacaatg gtattcacaa aattctgtgg aggcaatacc aatgtttatc tacccagttc 3841 cgcaaggcac atggtcgatt gaaatctctt gtgaaggcta ccaggcgacg tcaagcacca 3901 cggaccccca ccgggggaag tctgatggaa tgattgctta cgacgatgat caaaataagg 3961 tgtggaatat tggtgtacaa aataatgtaa ccatttccaa taacaaagcc gacgctgatt 4021 ggaaatacgg ccaccctgat ttaacaatca acggtgaacg ttttgaccag aagcaggtag 4081 tcgagaaaga tggaacagtt tcgtttcatt tagtaactac cgggcccaac gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagactg ccaagtacaa ctactgtata tcttatgggg 4201 cttggacaga ccgcgacatg gaatttggaa tggtttctgt tgttctcgat gaacatttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ctatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctat ccgtcgcttc agaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggtaaagag aagctacagg 4741 atgaagatcc aatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtct accttaagta 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtacgagc 4981 cagtgccaaa gtccgtttta gataggctta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatactt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga attaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS 5434 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 5434. ACCESSION 5434 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="5434" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Breeza, NSW" /collection_date="2015" /collected_by="Murray Sharman" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAATKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGSISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSDDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWTNIQ AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDDQN KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNYCISYGAWTDRDMEFGMVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFRPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGIGIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRLRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGSISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSDDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAVFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSKPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVTTNSRSTTRGTETPRLQAPSRSRSLS" BASE COUNT 1622 a 1183 c 1271 g 1383 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattt aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc tactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctaccga ggctctaatt aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcaacaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaagga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgaca tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcattctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acggacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gattcttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 aatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcagtattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaagc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aaatactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga cgaccaattc aaggtccact 3481 acaaggggaa cggagacgcc aagattgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc taaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgagagattc attgtgtaca 3661 ccggtacatt atcaacactc attagtgccc gacaaaactc agatagcata tcactctact 3721 cgatcagaga ccagagggtt agatatattg aggatgaaaa tgcgaactgg acgaacattc 3781 aagcgcaatg gtattcacaa aattctgtgg aggcaatacc gatgtttatc tacccagttc 3841 cgcaaggcac atggtcgatt gaaatctctt gtgaaggcta ccaggcgacg tcaagcacca 3901 cggaccccca ccgggggaag tctgatggaa tgattgctta cgacgatgat caaaataagg 3961 tgtggaatat tggtgtacaa aataatgtaa ccatttccaa taacaaagcc gacgctgatt 4021 ggaaatacgg tcaccctgat ttaacaatca acggtgaacg ttttgaccag aagcaggtag 4081 tcgagaaaga tggaacagtt tcgtttcatt tagtaactac cgggcccaac gcgagtttct 4141 ttttagctgg ccctgccgtt aaaaagactg ccaagtacaa ctactgtata tcatacgggg 4201 cttggacaga ccgcgacatg gaatttggaa tggtctctgt tgttctcgat gaacatttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc agaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggtaaagag aagttacagg 4741 atgaagatcc aatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tggcattaag cctagattaa aagatcagga tatgaggtct accttaagta 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagccccg aggtacgagc 4981 cagcgccaaa gtccgtttta gataggctta gacgtaggca agtctaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttca gttgcatatc caaagaataa ccctacactt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctgatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS 5436 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 5436. ACCESSION 5436 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="5436" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Edgeroi, NSW" /collection_date="2015" /collected_by="Murray Sharman" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLITATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPTVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPAPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPEGTWSIEISCEGYEVLSSTTDPHRGKSVGMIAYDDDQT KVWNVGAQNNVTISNNKADTGWKYGHPDLEINGEKFDQQQVVEKDGTVSFHLVTTGPN ASFFLVAPAVKKTAKYNYCISYGDWTDRDMEFGMVSVALDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPMTVHLVKAGDEESYATDQ TDDVEPTLLDADVMKFSGFVGARDRFSLQGAEPIGPGDRTSRRFRPSATLKAAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLHGEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGIKIKPRLKDQDMRSTLSTGTLKGGSLKSKKELPPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSLRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1633 a 1176 c 1269 g 1381 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatccctagt gttattgttg 61 gttatgttta acttcgatag cttaattaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgct tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aaacagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact ggggtcatgg 361 ccacaacctt gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggctaaccc tactgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt tcaaaagaag 541 gaggtacttt ccgccactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattatat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga tactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taaacttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacac 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttcccatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatataga ggctgccaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga agggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actatcaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcataacg 1321 cattggtagc ggttgaacgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttttcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccctagg acgttctccc ctgaagaact tgcagcgacg taccactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgatagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagataaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg aaaagaaaga tgaaaacaag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaaccg ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagttgtttg gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tggttttgct attggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 aatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttt aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc taaataggta gacggagaac cggggccgaa accaggtccg gcccccacac 3601 ccccaccagc accaccacct gagccgacac cggctaaaca tgaacgattc atcgtgtaca 3661 ctggcacatt gtcaacgctc atcagcgctc ggcaaaactc agacagtata tcactctact 3721 ctattagaga tcaaagagtt cgatatattg aagatgagaa cgcaaactgg acgaacatca 3781 aagcgcagtg gtattcacaa aattctgtgg aagccatacc aatgtttatc tacccagttc 3841 cagaaggtac atggtctatt gaaatctcat gtgaagggta tgaggtttta tctagcacca 3901 cggaccccca tcgggggaag tccgttggta tgattgcata tgatgatgat cagaccaaag 3961 tgtggaatgt tggtgcgcaa aacaacgtaa ccatatccaa taataaggca gacactggtt 4021 ggaagtacgg acacccagat ttagaaatca atggggagaa gttcgaccaa cagcaagtag 4081 tcgagaaaga tggaacagtt tcattccatt tagtaactac ggggcctaac gcgagtttct 4141 ttttggtggc tccagccgtc aagaaaacag ccaagtacaa ttactgtata tcatacggtg 4201 attggacaga tcgcgacatg gaatttggaa tggtctcagt tgctctcgat gaacatcttg 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcat attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cagacaattc tcctatgacc gttcaccttg 4441 taaaagcagg tgacgaggag agttatgcaa cagatcaaac agacgatgtt gagcccacac 4501 ttcttgatgc tgatgtcatg aagttctccg ggtttgttgg cgcgagggac aggttttcat 4561 tgcagggtgc cgaaccaatc ggcccaggtg acagaacctc ccgtcgcttc agaccgtctg 4621 ctacactaaa ggctgcgttc cattcgggac cgacccctga tgagttgtat aatgctggtg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctcccg gggcaaagag aagctgcacg 4741 gcgaagatcc aatggccaaa ctcgaaagag aggctgagga tttgaaacct gacagcgaca 4801 acgaagacaa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgaccctt 4861 ggtcgggcat taaaattaag cctagattaa aagaccaaga tatgaggtcc accttaagca 4921 ctggtacctt gaaaggaggt tcactgaaat cgaagaaaga actcccacca aggtttgaac 4981 cagcgccaaa gtctgtttta gatagaatta gacgtaggca agtctagtct tgcaccaaca 5041 tagaagtagt tagcgctttc cattattatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcta 5161 ctcccgtcgt atcactttcg gttgcatatc cagaaaataa ccctatattt aataactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggagg gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga aataacagcg taaacaaatt gttagtagct 5401 actccgtgaa aagagagtgg ctagtacgcc ccttagatgg cgttaaatga aagcgtagg // LOCUS 5481 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 5481. ACCESSION 5481 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="5481" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Colley Blue, NSW" /collection_date="2018" /collected_by="Murray Sharman" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQMQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDIAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLIQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRCSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFRPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT ATVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEATFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRSLMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1618 a 1177 c 1269 g 1395 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa atgcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tatagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcat tcaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgtcaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctccctt ctaagaaaga taaagaaggg aatgaattac cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cggtatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga agggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgtcccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtacaat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatacaatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agttaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 taccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaacattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggtcat taatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta cgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgact 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaggtag 4081 tcgagaaaga tggaacggtc tcgttccatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag atgttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc agaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aatgctggcg 4681 ctgaacaagc ggaccacgac ttcgaacatt ttgtctctcg aggcaaagag aaactacagg 4741 atgaggatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgcta ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgtaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS 5483 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 5483. ACCESSION 5483 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="5483" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Breeza, NSW" /collection_date="2018" /collected_by="Murray Sharman" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDATFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLNAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYTTDR SDDVEPILHDADVMKFSGFVGAKDRFMLQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGTEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1618 a 1184 c 1272 g 1385 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc ccctttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtggtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgcaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgacgcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atttacaacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaacaag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct taatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagttgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 cgattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatttcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggaccccca ccgggggaag tccgacggaa tgattgctta tgacgatgac caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caacaaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagctgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgctccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgacc gttcaccttg 4441 taaaagcagg tgacgaggag aattatacaa cagatcgctc ggacgatgtt gaacccatac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagac aggtttatgt 4561 tacagggtgc tgatccaatc ggccctggtg acaggtctgt ccgtcgcttc aaaccgtccg 4621 ccacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggca 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaggag aagctacagg 4741 atgaagatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgata 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagtatcaag cctagattaa aagatcagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtatgagc 4981 cagcgccaaa gtccgtctta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtaga tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct gggaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctacattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS 155MS 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 155MS. ACCESSION 155MS VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="155MS" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Spring Ridge, NSW" /collection_date="2015" /collected_by="Murray Sharman" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVCQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFRDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWTNIQ AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDDQN KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNYCISYGAWTDRDMEFGMVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPVPKSVL DRFRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTNSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1625 a 1190 c 1267 g 1377 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgcctt cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc tactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tctgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagcttcctt ctaaaaaaga taaagaagga aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 atccgcaaca ataatatgag gttgatagac ggctctgatg tttcccatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgaca tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgtcgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcca atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg taccactctg 1501 gtaagagaac gttgtataat gctgctgtcc aaagccttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgtaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 tgagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atttataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaag atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcgcttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttagagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tgcctgttct cgatgctttt cacaaatgcc tatacaaagc atcagggtac aagaaagtat 2461 cgcaggaagc gattgagagg atagtctcct tcggaacgca ggacaagctc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 aatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccaattc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgagagattc attgtgtaca 3661 ccggtacatt atcaacactc attagtgccc gacaaaactc agatagcata tcactctact 3721 caatcagaga ccagagggtt agatatattg aggatgaaaa tgcgaactgg acgaacattc 3781 aagcacaatg gtattcacaa aattctgtgg aggcaatacc aatgtttatc tacccagttc 3841 cgcaaggcac atggtcgatt gaaatctctt gtgaaggcta ccaggcgacg tcaagcacca 3901 cggaccccca ccgggggaag tctgatggaa tgattgctta cgacgatgat caaaataagg 3961 tgtggaatat tggtgtacaa aataatgtaa ccatttccaa taacaaagcc gacgctgatt 4021 ggaaatacgg ccaccctgat ttaacaatca acggtgaacg ttttgaccag aagcaggtag 4081 tcgagaaaga tggaacagtt tcgtttcatt tagtaactac cgggcccaac gcgagtttct 4141 ttttagctgg ccctgccgtt aagaagactg ccaagtacaa ctactgtata tcttacgggg 4201 cttggacaga ccgcgacatg gaatttggaa tggtctctgt tgttctcgat gaacatttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc aaaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaagag aagctacagg 4741 atgaagatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtacgagc 4981 cagtgccaaa gtccgtttta gataggttta gacgtagaca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaaaccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatactt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttcca aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtt 5401 actccatgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS 203MS 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 203MS. ACCESSION 203MS VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="203MS" /host="Medicago polymorpha" /db_xref="taxon:12049" /country="Australia: Edgeroi, NSW" /collection_date="2013" /collected_by="Murray Sharman" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGYGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHSYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWTNIQ AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDDQN KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNYCISYGAWTDRDMEFGMVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRMASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTNSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1629 a 1192 c 1262 g 1376 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgcctt cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtatggc 481 gataaatcac cttccaatcc tactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacattcat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatccg tccgccaaat ggttaccgag 721 gtaaatgtca tccccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagcttcctt ctaagaaaga taaagaagga aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 atccgcaaca ataatatgag gttgatagac ggctctgatg tttcccatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgaca tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta ttcctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgtcgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg taccactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagccttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaag atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcgcttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tgcctgttct cgatgctttt cacaaatgcc tatacaaagc atcagggtac aagaaagtat 2461 cgcaggaagc gattgagagg atagtctcct tcggaacgca ggacaagctc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 aatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggatggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccaattc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaaccc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgagagattc attgtgtaca 3661 ccggtacatt atcaacactc attagtgccc gacaaaactc agatagcata tcactctact 3721 caatcagaga ccagagggtt agatatattg aggatgaaaa tgcgaactgg acgaacattc 3781 aagcacaatg gtattcacaa aattctgtgg aggcaatacc aatgtttatc tacccagttc 3841 cgcaaggcac atggtcgatt gaaatctctt gtgaaggcta ccaggcgacg tcaagcacca 3901 cagaccccca ccgggggaag tctgatggaa tgattgctta cgacgatgat caaaataagg 3961 tgtggaatat tggtgtacaa aataatgtaa ccatttccaa taacaaagcc gacgctgatt 4021 ggaaatacgg ccaccctgat ttaacaatca acggtgaacg ttttgaccag aagcaggtag 4081 tcgagaaaga tggaacagtt tcgtttcatt tagtaactac cgggcccaac gcgagtttct 4141 ttttagctgg ccctgccgtt aagaagactg ccaagtacaa ctactgtata tcttacgggg 4201 cttggacaga ccgcgacatg gaatttggaa tggtctctgt tgttctcgat gaacatttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcaaggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc aaaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaagag aagctacagg 4741 atgaagatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa tgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtacgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtagaca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaaaccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatactt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtt 5401 actccatgaa aagagagtga ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L42 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L42. ACCESSION L42 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L42" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFRPSATLKSAFHSGP TPDELYNAGVEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEATFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLLMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1616 a 1176 c 1272 g 1395 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt tcaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctccctt ctaagaaaga taaagaaggg aatgaattac cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cggtatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga agggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgtcccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaacattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat taatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta cgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaggtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc agaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aatgctggcg 4681 ttgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaagag aaactacagg 4741 atgaggatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L43 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L43. ACCESSION L43 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L43" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSCGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPSP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKSLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFRPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1614 a 1172 c 1274 g 1399 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggtcaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgct tttgttgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt tcaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat acgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat taagagggca 661 gcagcaaaac aagccaaggc ggccaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagagggg aatgaattgc cacctgagcc taagctcaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aatttaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccgactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttgtggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgtttg gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 cctcaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caatccgaga tcagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcaatt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttcttgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aatcgctaag accaggtcac attagcgtca 4321 atcgttctca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaagaagata 4381 atagtagtag tgatagcaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc agaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aatgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaagag aagctacagg 4741 atgaagatcc aatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga catgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagctcca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L45 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L45. ACCESSION L45 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L45" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEFKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLIQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENHSTDR SDDVEPSLHDADVMKFSGFVGAKDRFALQGADPIGPGNRSVRRFRPSATLMSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLIHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1617 a 1167 c 1273 g 1402 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaatt caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcat tcaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taaacttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagaggac gttgtataat gctgctgttc aaagtcttaa acgcaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg atattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagtata tccaaagacg ctcattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagttgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accgatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct catacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttga 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcacaatg gtattcgcag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcaatt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tatggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aatcactcaa cagatcgatc ggacgatgtt gaaccctcac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggta acagatctgt ccgtcgcttc agaccgtctg 4621 caacattgat gtctgcgttc cattcgggac cgactcctga tgagttgtac aatgctggcg 4681 ctgaacaagc ggatcacgac ttcgagcact ttgtctctcg aggcaaagag aaactacagg 4741 atgaggatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattattatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaat aaaaagccgg taatctcggg gagatgtcaa 5161 ctcccgccgt atcactttcg gttgcatatc cagaaaataa ccctatattt aataactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggagg gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga aataacagcg taaacaaatt gttagtagct 5401 actccgtgaa aagagagtgg ctagtacgcc ccttagatgg cgttaaatga aagcgtagg // LOCUS L46 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L46. ACCESSION L46 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L46" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYTTDR SDDVEPILHDADVMKFSGFVGAKDRFMLQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGTEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGINIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1621 a 1178 c 1270 g 1390 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctacaacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 cgattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatttcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagctgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgctccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgacc gttcaccttg 4441 taaaagcagg tgacgaggag aattatacaa cagatcgatc ggacgatgtt gaacccatac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagac aggtttatgt 4561 tacagggtgc tgatccaatc ggccctggtg acaggtctgt ccgtcgcttc aaaccgtccg 4621 ccacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aatgctggca 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaggag aagctacagg 4741 atgaagatcc aatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgata 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat taatatcaag cctagattaa aagatcagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtatgagc 4981 cagcgccaaa gtccgtctta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtaga tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct gggaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctacattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L51 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L51. ACCESSION L51 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L51" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Glen Innes, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHKYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPVLSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGPGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYTTDR SDDVEPILHDADVMKFSGFVGAKDRFILQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGTEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGPGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1623 a 1180 c 1270 g 1385 t 1 others ORIGIN 1 acaagaacct cctcccggca tctycgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtggaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtact ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacataaat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ctttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atttacaacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac cagtactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggacc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aaatactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 cgattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatttcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagctgg ccctgccgtt aagaagattg ccaagtataa cttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgctccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgacc gttcaccttg 4441 taaaagcagg tgacgaggag aattatacaa cagatcgatc ggacgatgtt gaacccatac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagac aggtttatat 4561 tacagggtgc tgatccaatc ggccctggtg acaggtctgt ccgtcgcttc aaaccgtccg 4621 ccacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aatgctggca 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaggag aagctacagg 4741 atgaagatcc aatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgata 4801 acgaagataa cgattatgat cgaaccgctc ccatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagtatcaag cctagattaa aagatcagga tatgaggtcc accttaagca 4921 ctggcacctt aaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtatgagc 4981 cagcgccaaa gtccgtctta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtaga tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct gggaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctacattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L56 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L56. ACCESSION L56 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L56" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Matheson, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKTGDEENYTTDQ SDDVEPILHDADVMKFSGFVGAKDRFMLQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGTEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGINIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1621 a 1180 c 1270 g 1388 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggat ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcggaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcccgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc tccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caacaaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atttataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gagctctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg cccaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatataaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaaattgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 cgattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtatatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggcta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgctccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgacc gttcaccttg 4441 taaaaacagg tgacgaggag aattatacaa cagatcaatc ggacgatgtt gaacccatac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagac aggtttatgt 4561 tacagggtgc tgatccaatc ggccctggtg acaggtctgt ccgtcgcttc aaaccgtccg 4621 ccacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggca 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaggag aagctacagg 4741 atgaagatcc aatggccaag ctcgaaagag aggctgagga tttgaaacct gatagtgata 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat taatatcaag cctagattaa aagaccagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtatgagc 4981 cagcgccaaa gtccgtctta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtaga tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct gggaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctacattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgacgg cgttaaatga aagcgtagg // LOCUS L60 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L60. ACCESSION L60 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L60" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Shannon Vale, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDIAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLIQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFVLQGADPIGPGDRSVRRFRPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEATFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRSLMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1618 a 1173 c 1270 g 1398 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgactttga tatagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcat tcaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgtcaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctccctt ctaagaaaga taaagaaggg aatgaattac cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cggtatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga agggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgtcccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattta 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 taccatagta gttatagtac tagtagtagt tagttgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaacattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggtcat taatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta cgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaggtag 4081 tcgagaaaga tggaacggtc tcgttccatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgtgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc agaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aatgctggcg 4681 ctgaacaagc ggaccacgac ttcgaacatt ttgtctctcg aggcaaagag aaactacagg 4741 atgaggatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L69 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L69. ACCESSION L69 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L69" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDQSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGLIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQGEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGINIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPVPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPREALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEATFPEDQARLIHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1614 a 1178 c 1273 g 1394 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttgttgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgaatg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gatcaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt tcaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctccctt ctaagaaaga taaagaaggg aatgaattac cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cggtatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga agggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgtcccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccata ctgaaggagt 1921 tgttacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtc atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcgcg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaacattt 3121 ccggaggatc aggcaaggct catacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggac tgattgctta cgatgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc aaaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaagag aagctacagg 4741 gtgaagatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat taacattaag cctagattaa aagatcagga tatgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagtccca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatattt gacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctagtacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L72 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L72. ACCESSION L72 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L72" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYRRLLADGAPLGSWPQP CAPQGSFRSSDDFDAAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPAKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVYIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTHKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGARDRFALQGADPIGPGDRSVRRFRPSATLKSAFHSGP TPDELYSAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPASRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIYPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSTASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1619 a 1175 c 1272 g 1393 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgcta 61 gttatgttta acttcgatag cttagttact gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tgtttgctgc acatgacgct tttgtcgaca tgtgttatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata taggcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgcagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt tcaaaagaaa 541 gaggtacttt ccgctaccga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctccctg ctaaaaaaga taaagaaggg aatgaactgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatacat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggttctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa aagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta ttttcgcaaa tctattgtgg 1441 ataaggttgg ttgcccaagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagccttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagaa aacaaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtctata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatatat 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caacggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcaatt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccggggaaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg tcatcctaat ctaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggacaga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaggata 4381 atagtagtag tgatagtaat acacataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctagagat aggtttgcgt 4561 tgcagggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc agaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac agtgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaagag aaactacagg 4741 atgaggatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcttca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L77 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L77. ACCESSION L77 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L77" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYTTDQ SDDVEPILHDADVMKFSGFVGAKDRFMLQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGTEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGINIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1619 a 1179 c 1273 g 1388 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggat ccacaaccct ctttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcctgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac agaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcggaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc tccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caacaaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atttataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gagctctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg cccaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 cgattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggcta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgctccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgacc gttcaccttg 4441 taaaagcagg tgacgaggag aattatacaa cagatcaatc ggacgatgtt gaacccatac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagac aggtttatgt 4561 tacagggtgc tgatccaatc ggccctggtg acaggtctgt ccgtcgcttc aaaccgtccg 4621 ccacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggca 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaggag aagctacagg 4741 atgaagatcc aatggccaag ctcgaaagag aggctgagga tttgaaacct gatagtgata 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat taatatcaag cctagattaa aagaccagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtatgagc 4981 cagcgccaaa gtccgtctta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtaga tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct gggaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctacattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg agtaaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgacgg cgttaaatga aagcgtagg // LOCUS L87 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L87. ACCESSION L87 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L87" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPSKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYSTDR SDDVEPTLHDADVMKFSGFVGAKDRFALQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGAEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APVFNPADGDPWSGISIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRFEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEATFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLLMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1620 a 1177 c 1268 g 1394 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcatctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt tcaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctccctt ctaagaaaga taaggaaggg aatgaattac cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cggtatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcgaaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga agggctctgc tccgactctg gttttgaatc cccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgaacgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga atcttctcta atcttgatta tttccgcaaa tctattgtgg 1441 acaaggttgg ttgtcccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atctataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aacaaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg ctcaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaacattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat taatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 caattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcacaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggtta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgttccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgact gttcaccttg 4441 taaaagcagg tgacgaggag aattactcaa cagatcgatc ggacgatgtt gaacccacac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagat aggtttgcgt 4561 tgcaaggagc tgatccaatc ggccctggtg acagatctgt ccgtcgcttc aaaccgtctg 4621 caacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggcg 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaagag aagctacagg 4741 atgaagatcc gatggccaaa ctcgaaagag aggctgagga tttgaaacct gatagtgaca 4801 acgaagataa cgattatgat cgaaccgctc ctgtgttcaa cccagcagat ggtgacccct 4861 ggtctggcat tagcattaag cctagattaa aagatcagga tatgaggtct accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggttcgagc 4981 cagcgccaaa gtccgtttta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtagt tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct ggtaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctatattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgatgg cgttaaatga aagcgtagg // LOCUS L59 5459 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L59. ACCESSION L59 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5459) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5459 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L59" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Matheson, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 64..2747 /gene="RdRp" CDS join(64..1147,1147..2745) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVTATARVVKDFISFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQYSTAEIDVQLAEHEYKRLLADGAPLGSWPQP CAPQGSFRSSDDFDVAARAAEANPIVDPQPSLIKGCGDKSPSNPTRIEKFINLVQKKE VLSATEALIKHTYEEQIGESPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSKSVRQMVT EVNVIPDFISCCDVVQVDTGEKLPTKKDKEGNELPPEPKLKMFRRVKPEHVGDARKYI CQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTEMLIKYAMVMVPLPRKSD IEAAKIVQSPAARQIRHELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGAIPR KSRSTINYLSQFSLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNIPGIFSN LDYFRKSIVDKVGCPRTFSPEELAATYHSGKRTLYNAAVQSLKRKAVERSDANVTAFL KMEKHLMCKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFDSPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFNDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKSLGVKAELCNNG DDCVIICEKKDENKFSHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKQESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAFHKCLYKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESFGVDPQTQVLVERYFDNL QVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2825..2966 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYRFLSGFAIGFIASIPISILAIYFVYLKISKNLRGIINEYGRG" CDS 2955..5025 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3555..3557,aa:OTHER) /product="P3/P5" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRVRYIEDENANWAYIK AQWYSQNSVEAVPMFIYPVPQGTWSVEISCEGYQATSSTTDPHRGKSDGMIAYDDDQT KVWNVGVQNNVTITNNKADADWKYGHPNLTINGERFDQQQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKIAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRSHRLQEMPPMREDFSEEDNSSSDSNTYKTDNSPLTVHLVKAGDEENYTTDR SDDVEPILHDADVMKFSGFVGAKDRFMLQGADPIGPGDRSVRRFKPSATLKSAFHSGP TPDELYNAGTEQADHDFEHFVSRGKEKLQDEDPMAKLEREAEDLKPDSDNEDNDYDRT APMFNPADGDPWSGINIKPRLKDQDMRSTLSTGTLKGGSLKPKKEPAPRYEPAPKSVL DRIRRRQV" gene 2955..3557 /gene="CP" CDS 2955..3555 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVTIQRRRNRRAARSAPRVQLMAIPTTTGATQRRRRQRR RRRNNRGGGNISGGSGKAHTFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYSEIQSLLNKFSITKSGSKRFPTKVIN GLEWHDTSEDQFKVHYKGNGDAKIAGSFKITIVVLTQNPK" gene 2971..3538 /gene="MP" CDS 2971..3538 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSQYNDDAIVEPHEALHEFSSWLFQRPPAQHSAEEDNDDEGEII EEEAIFPEDQARLTHSSFRRTASMAVPRELSLSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTASTPKFNRYSINSQLQRAVRNDSPPRLSMASNGM IPVKTSSRSTTRGTETPRSQAPSRSRSLS" BASE COUNT 1619 a 1180 c 1273 g 1387 t ORIGIN 1 acaagaacct cctcccggca tcttcgtatc gtgtttgagg aatctctagt gttattgtta 61 gttatgttta acttcgatag cttagttaca gccaccgcta gggtggttaa agattttatt 121 tctttttgct ataatagggc acggcatgtt tattatgccc taaaaagatg gctctgggaa 181 ctccagggtg tatttgctgc acatgacgcc tttgtcgaca tgtgctatga cgccatgtat 241 ggcgttgagg agttcgagtg ggaactgcaa aagcagtatt ccactgcaga gattgacgtg 301 caattagctg agcacgaata caagcgccta cttgccgatg gcgctccact agggtcatgg 361 ccacaaccat gtgcgcccca aggctcattc aggtcgtctg acgacttcga tgtagctgct 421 agggctgctg aggcaaaccc tattgtggac ccacaaccct ccttgattaa agggtgtggc 481 gataaatcac cttccaatcc cactaggatt gagaagttca tcaacctcgt ccaaaagaag 541 gaggtacttt ccgctactga ggctctaatc aaacatacat atgaagagca aatcggtgaa 601 tcaccattcg gaaagtggtt caacactctg cccaatagaa tgaattacat aaagagggca 661 gcagcaaaac aagccaaggc ggctaaacga tctaaatctg tccgccaaat ggttaccgag 721 gtaaacgtca ttcccgactt tatctcttgc tgtgatgttg tccaggtgga cactggtgaa 781 aagctcccta ctaagaaaga taaagaaggg aatgaattgc cacctgagcc taagcttaaa 841 atgtttagaa gggtgaagcc tgagcatgtt ggtgatgcca ggaaatatat ctgtcaacat 901 attcgcaaca ataatatgag gttgatagac ggctctgatg tttctcatgc aaccatcaac 961 cgatatgcgc tcaagttttg cgaagacttg gagcttgata tgaccagcac tgaaatgctc 1021 atcaaatatg ctatggtcat ggtcccactc cctcggaaat ctgatattga ggctgctaaa 1081 atcgtccaat ctcctgctgc caggcaaata aggcatgagt tgggcgtgct taacgccgag 1141 gttttttaga ggggctctgc tccgactctg gttttgaatc tccgttttct attttggggt 1201 tgccagagat cgtggtgcga agtggggcta tccctaggaa gagtcgtagt actattaatt 1261 atttgagtca gtttagttta ggtctagatt accaatgccc taatcccagt ttgcacaacg 1321 cattggtggc ggttgagcgt agagtgttca ccgttgggaa aggaaatgag gtagtgctac 1381 cttacaagaa cataccagga attttctcta atcttgatta tttccgcaaa tctattgtgg 1441 ataaggttgg ttgccccagg acgttctccc ctgaagaact tgcagcgacg tatcactctg 1501 gtaagagaac gttgtataat gctgctgttc aaagtcttaa acgtaaggct gttgagagga 1561 gtgacgcgaa tgtgacagct ttccttaaga tggagaagca tcttatgtgt aagaagatag 1621 cacccaggtt gatatgtccc cgcaacaaaa gatataatgt tgaattggga cgtcgattga 1681 aattcaatga gaagaaattc atgcatgcaa tcgactcaac ttttgattcc ccaactgtat 1741 taagtggata tgacagtttt aaagttggga agataatagc caataaatgg tcgaaattca 1801 aacgaccagt tgcaataggt gttgatgcaa gcagatttga tcaacatgtg ggggtagaag 1861 cactccaatg ggagcactca atttataacg gtgcattcaa tgatcccatt ctgaaggagt 1921 tgctacactg gcaaacagag aataaaataa tgctgtttgt tgaagacaaa atcctcaagt 1981 tcaaggtcaa aggacacaga atgtccggtg acattaacac ctcttctggc aacaaattaa 2041 tcatgtgtgg tatgatgcac tactacttca agtcacttgg agtcaaagcc gaactctgca 2101 ataacggcga tgattgtgtt atcatatgcg agaagaaaga tgaaaataag ttctcacaca 2161 tgcatagctg gtttaaagac tatgggtttg acatgcagat tgagactcct gtctacaaga 2221 ttggacagat agagttttgt caaagtaaac cagttaaaat taatggctat tatagaatgg 2281 tgcgtaaaca agaaagcata tccaaagacg cccattccct catttccatg gcatcagctg 2341 aagatgtcaa aacatttatg agtgccaccg cccaatgcgg gatgatcttg aatagtggtg 2401 tacctgttct tgatgctttt cacaaatgcc tatacaaagc atcggggtac aagaaagtat 2461 cgcaggaagc gatcgagagg atagtctcct tcggaacgca ggacaaactc gggctcagga 2521 aagagcgagt tgaagaacca ataacgatgg ataataggtt gagttattgg gagtcctttg 2581 gggttgaccc gcagacacag gtccttgtcg aacgatattt tgacaatctg caggtccata 2641 tcgaaccccg cggcgtaaag agattgacgc ctttactcga taaaactttg ctttcaattg 2701 cgagtgtagc acgaacatct gtgtcgctac caatactttc aaaatagaag cactcataat 2761 caccatagta gttatagtac tagtagtagt tagctgttta gattacgcta gttcgtacaa 2821 gtaagttgca acggattacc gatttttatc tgggtttgct atcggcttta tagctagcat 2881 accaatatca atattggcaa tttactttgt ttacctaaaa atttcaaaga atctgagagg 2941 gatcatcaac gaatatggtc gcggttagca atgtcacaat acaacgacga cgcaatcgta 3001 gagccgcacg aagcgctcca cgagttcagc tcatggctat tccaacgacc accggcgcaa 3061 cacagcgcag aagaagacaa cgacgacgaa ggagaaataa tagaggagga ggcaatattt 3121 ccggaggatc aggcaaggct cacacattcc agttttcgaa ggacggcatc aatggcagtt 3181 ccaagggaac tatcactttc gggccgtctt tatcagagtg caagccactc tctgatggaa 3241 tactcaaggc ctaccatgaa tataagatca cgagtatctt attacagttc atctccgagg 3301 cctcttccac ctcgtcaggc tccatcgctt atgagcttga cccacactgc aagtactccg 3361 aaattcaatc gctactcaat aaattctcaa ttacaaagag cggttcgaaa cgattcccca 3421 ccaaggttat caatggcctc gaatggcatg ataccagtga agaccagttc aaggtccact 3481 acaaggggaa cggagacgcc aagatcgcag gctccttcaa gatcacgatc gttgtcttaa 3541 ctcagaatcc caaataggta gacggagaac cggggccgaa accaggtccg gaccccacac 3601 ccccaccagc accaccacct gaaccgacgc cagccaaaca tgaaagattc attgtttaca 3661 ccggtacact gtcaacactc atcagtgctc gacagaactc agacagtata tcactctact 3721 cgattcgaga ccagagggtc agatatattg aagatgagaa tgcaaactgg gcgtacatca 3781 aagcgcaatg gtattcacag aattctgtgg aagcagtacc aatgtttatc tacccagttc 3841 cgcaaggtac ttggtcagtt gaaatctcat gtgaaggcta ccaggcaaca tcgagcacca 3901 cggatcccca ccgggggaag tccgatggaa tgattgctta tgacgatgat caaaccaagg 3961 tgtggaatgt tggtgtgcaa aataatgtaa ccataaccaa caataaagcc gatgctgatt 4021 ggaaatacgg ccatcctaat ttaacaatta atggtgaacg ttttgaccag cagcaagtag 4081 tcgagaaaga tggaacggtc tcgtttcatt tagtaactac tgggcccaat gcgagtttct 4141 ttttagccgg ccctgccgtt aagaagattg ccaagtataa tttctgtata tcatatggag 4201 cttggactga tcgcgatatg gagtttggtt tggtatctgt tgttctcgat gaacacttag 4261 agggtgttag aggttctcag tacgttagga aaacgctaag accaggtcac attagcgtca 4321 atcgctccca ccgattgcaa gaaatgcctc ccatgcgaga ggatttcagt gaggaagata 4381 atagtagtag tgatagtaat acatataaaa cggacaactc tcccttgacc gttcaccttg 4441 taaaagcagg tgacgaggag aattatacaa cagatcgatc ggacgatgtt gaacccatac 4501 tccatgatgc tgatgtcatg aagttctccg ggtttgttgg agctaaagac aggtttatgt 4561 tgcagggagc tgatccaatc ggccctggtg acaggtctgt ccgtcgcttc aaaccgtctg 4621 ccacattgaa gtctgcgttc cattcgggac cgactcctga tgagttgtac aacgctggca 4681 ctgaacaagc ggatcacgac ttcgaacact ttgtctctcg aggcaaggag aagctacagg 4741 atgaagatcc aatggccaag ctcgaaagag aggctgagga tttgaaacct gatagtgata 4801 acgaagataa cgattatgat cgaaccgctc ctatgttcaa cccagcagat ggtgacccct 4861 ggtctggcat taatatcaag cctagattaa aagaccagga tatgaggtcc accttaagca 4921 ctggcacctt gaaaggtggt tcactgaaac cgaagaaaga accagcccca aggtatgagc 4981 cagcgccaaa gtccgtctta gataggatta gacgtaggca agtttaatct tgcaccaata 5041 tagaagtaga tagcgctttc cattgttatt tataggagcc aaatttggtg agtcccgatc 5101 gatggatcct gggaaacagg ttcggtgaac aaaaagccgg taatctcggg gagatgtcag 5161 ctcccgtcgt atcactttcg gttgcatatc caaagaataa ccctacattt aacaactaaa 5221 aattagatag tttccgcctc tcttgcaaac cacatgcaag atgcgagctt gtggagagta 5281 ccttcccttg aataaggaag gttgaaagtt tctggttccc aaaccattga tcaaaattgg 5341 ctctgcttct ggtgattaca ctgccgctga atcaacagcg taaacaaatt cctattggtc 5401 actccgtgaa aagagagtgg ctaatacgcc cctttgacgg cgttaaatga aagcgtagg // LOCUS BC2020 5604 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate BC2020. ACCESSION BC2020 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5604) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5604) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5604 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="BC2020" /host="Lens Culinaris" /db_xref="taxon:12049" /country="Australia: Grass Patch, WA" /collection_date="2019" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMVPEPSLIKGYGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKFKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTVMLVDYAMTMVPIPLKND VERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENKIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5137 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRSHRLQDNFVPAEYVSDEDSSSSSSIASNRPSTPDNDSDAQFVESMKGKLPS QTKLPPKGFLSRLSAKEKKEISNSKPSNVEGLVGPLVAAYGYPSQTGVYDAAGEILQA KEAAENLADLERDLKEIDKLEPPDIIVQEEIPDFVPPSEKTLKEDDPDYVSSIWHNAD QAELVSTYEPPDWSRPANELGDPSKKARTLKGTLSKLGGSLRSGESSLRGSLRKTQDQ TDLDNKLSKLNVIQRSQYQRILNNMGATRARAYIDGLDLG" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV FYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFSTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1688 a 1201 c 1291 g 1424 t ORIGIN 1 acaagatcca cctcccggca tcttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggtcccag aaccctcctt 481 gattaaaggg tatggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agaacatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatctgtg atgttgttca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa tttaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ctacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgta atgctggttg attatgccat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc ttgattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caacgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata aaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tattttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt ctcaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag acaaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaagacca 4321 ggtcatgtta gcgtcaatcg atctcaccga ttgcaagaca atttcgttcc ggcggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtatagcta gtaatcgacc atcaactccg 4441 gataatgata gtgacgccca atttgtagaa tctatgaaag gtaagcttcc ttctcagacg 4501 aagctcccac ctaaagggtt cctatcacgg ttaagcgcaa aagagaaaaa agaaatctct 4561 aattctaaac cctcgaatgt cgaaggcctg gttggtcctc ttgttgcagc ttatggatac 4621 ccatctcaaa cgggtgttta tgacgcagca ggagagatac tgcaagctaa agaagctgca 4681 gaaaacctgg cagacttaga gagggactta aaggagattg acaagcttga accaccggat 4741 ataattgtcc aggaagaaat ccctgacttt gtgccgccat cggagaagac attaaaggaa 4801 gatgatcccg attatgtatc ttcgatttgg cacaatgccg accaggctga attggtctca 4861 acttatgagc ctccagactg gagtcggcca gctaatgagt taggtgatcc atctaagaag 4921 gcacgaacac tcaaaggtac tctctccaaa ttagggggtt ctttaaggag tggtgaatcg 4981 tcgcttagag gtagcctccg caagacgcaa gatcaaactg atctagataa caagttatcc 5041 aaactcaatg ttatccagag atcccaatac cagaggattc tcaataacat gggtgcaacg 5101 cgcgcgcggg catatattga tggtttagat ttaggttagt ataatagaag taagcgtgcg 5161 ctttccatca cattaggtcg gagccaaact tggtgagttg ctgttgtggg atcctgggaa 5221 acaggttcgg tgaacaaaaa gccggtaatc tcgggcgttc gtcagacgcc gccgtatcaa 5281 cacagtatgc atatccatca ataacccgac ttctagtgac taaaaattag agttagtttc 5341 cgcctctctt gtaaaccaca tacaagatgc gagcttgtgg agagtaccat cccttgaata 5401 aggatggttg aaagtttctg gttccaatcc attgatcaaa attggcactg cttctggtga 5461 ttacactgcc gctggtgata cagcgtaaac aatttctgct acttgctcga cgaaatgaga 5521 gtaactagta aagacttgcc gttcacatgt ttatttgacg gtactaggct tcgtcccttt 5581 gatgacgtta aatgaaagcg gagt // LOCUS 3342 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 3342. ACCESSION 3342 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="3342" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Green Range, WA" /collection_date="2019" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVNTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND VERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKYP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYTPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMHARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1705 a 1202 c 1286 g 1416 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtgaatact ggtgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tattcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctc 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccaat actttcaaaa 2761 tagaagcact catagtaacc atagtagtgt tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gataatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg catccaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagta tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaatt agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacac acccccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcatgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc attgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS 5432 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 5432. ACCESSION 5432 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="5432" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Edgeroi, NSW" /collection_date="2013" /collected_by="Murray Sharman" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKVGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKLP MQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSETGVYDAARAILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGEMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQEPLMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1695 a 1202 c 1295 g 1417 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttgtttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtatcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtctttg atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tcccccccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaagatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaaaattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct atcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gtaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgat agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggtgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcaca aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgatcctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcctctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcacaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agccattaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cggttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagatcgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgatgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa aaccctcgaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgg aaacaggtgt ctatgatgca gctagagcga tcctgcaagc taaagaagct 4681 gcagagaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctgaataa cctaggggaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgc 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagctag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccggtca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS 5435 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 5435. ACCESSION 5435 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="5435" /host="Lens culinaris" /db_xref="taxon:12049" /country="Australia: Breeza, NSW" /collection_date="2015" /collected_by="Murray Sharman" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAVQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGASPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMNKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDESKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMM RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVASQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDDDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPTYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRAREYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVASQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHHNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1702 a 1193 c 1291 g 1423 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttga tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtatt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagttcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcattcc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctcttaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaaaattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagcttcacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattatca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctaaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgaataaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ctggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaaaac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 agtaaatttc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatgatgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtgcc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttgttagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcatcacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgagga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggtacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggctgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagttga gaaagatgga acagtttcgt ttcatttagt gactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agacagccaa gtataacttc 4201 tgtatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggatgatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacacag 4501 tcgaagcttc cacctaaagg attcctgtca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacaatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccaacttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagaatacat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatgcaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS 6091 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 6091. ACCESSION 6091 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="6091" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Esperance, WA" /collection_date="2017" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND VERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLQDSFTSVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKYP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDATREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPEKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVVQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1699 a 1201 c 1293 g 1416 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttga tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgcgg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc agactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggttaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagctgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg catccaccga ttgcaagaca gtttcacatc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagta tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatc 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcttatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca accagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acccccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctgag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttgttca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaag ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 tctttgatga cgttaaatga aagcggagt // LOCUS 6692 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 6692. ACCESSION 6692 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="6692" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Mt Barker, WA" /collection_date="2017" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKLMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND VERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLHDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKYP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DRAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNDLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQEPSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1696 a 1206 c 1294 g 1413 t ORIGIN 1 acaagatcca cctcccggca tcttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgcgg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacat gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc agactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaactga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgtgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcaattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctactata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcaca aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acctctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ttttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agccatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acagtggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttga ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg catccaccga ttgcatgaca gtttcacgcc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagta tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acccccgatt tggcgcaatg ccgaccgggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaatga cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS 6694 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 6694. ACCESSION 6694 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="6694" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Narrikup, WA" /collection_date="2017" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSNAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAGSKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLIDYAMTMVPIPLKND VERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGHIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPVTMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFVYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKYP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRMLNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSNFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1701 a 1199 c 1292 g 1417 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaaa 301 tgccgaacac gacgtgttaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctggtt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctgattg attatgctat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctgcctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcgct ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggtgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca tagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acatatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccagtaa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccaatt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttgtttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccatcgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg catccaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagcatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagta tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acccccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa tgctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacag gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcaatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS 6931 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 6931. ACCESSION 6931 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="6931" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Gairdner, WA" /collection_date="2017" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND VERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVSTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKYP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKTGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMHARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVSTQNPK" gene 2987..3521 /gene="MP" CDS 2987..3521 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPK" BASE COUNT 1704 a 1206 c 1289 g 1410 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcattaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa cagttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catcgaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaattat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagc ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tagcaggctc cttcaagatc 3541 acgatcgatg tctcaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt gtcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg catccaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagta tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acccccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aagacaggaa cactcaaggg aactctctca aaactaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcatgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc tcgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS 2MS 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 2MS. ACCESSION 2MS VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="2MS" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Warwick, QLD" /collection_date="2013" /collected_by="Murray Sharman" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKRFSSAEHDVLIAKHEFERLLRDGAPLKAWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLKGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILDNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHCNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEVIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQEPSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1694 a 1202 c 1293 g 1420 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac ggttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag gcatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtctttg atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtt acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaattat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataagttcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattaca gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcaca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattgcaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcacaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggta atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agccatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acattaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cggttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcactcc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa aaccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagagaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgcctc ctccggagag ggtgattcag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta aaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctagataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgc 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS 227MS 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate 227MS. ACCESSION 227MS VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="227MS" /host="Cicer arietinum" /db_xref="taxon:12049" /country="Australia: Pilton, QLD" /collection_date="2013" /collected_by="Murray Sharman" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGNFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLSSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPETKLKMMRRVRPEYYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPSP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSNFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1704 a 1198 c 1287 g 1420 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gatgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc aatttccggt cgtctgatga 421 cttccaggta gcagctcaag aggctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgtcct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaaaccaaa ttaaaaatga tgagaagggt caggcctgag tattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagttttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gagcgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggtgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcattact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtgtcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccaat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg actacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccaatt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccctc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactatca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ccgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaaactcc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc cccttgttgc agcctatgga 4621 tacccgtctc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat tgatggttta gatttagatt aatatagtag aagtaagtgc 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt actatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cttttgatga cgttaaatga aaacggagt // LOCUS KF20 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate KF20. ACCESSION KF20 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="KF20" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Scott River, WA" /collection_date="2020" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSNAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRQERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIR AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTISFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPDRVIQEDDPGYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QIDLDNKLSKLSVIQRSQYQRILNNLGKMRAREYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSKPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1703 a 1200 c 1286 g 1420 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaaa 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc ttcctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcatattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccccc tcaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcaattt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tataatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgta acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggcaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt tctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaagcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga atatcagagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acaatctcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg cattcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgcctc ctccggacag ggtgatccag 4801 gaagacgatc ctggttacat acccccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ttgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagaatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacaaaggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggtttc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS L49 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L49. ACCESSION L49 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L49" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Glen Innes, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLKDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPNRMNYIKRAAAKQAKAAKRSNSVRQMVN EVNVIPDFISICDVVLVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISYLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTISFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QNDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLN" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFNSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSKPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSLQEPSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1709 a 1198 c 1289 g 1413 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggttaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa aggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtctttg atggatccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccca atagaatgaa 661 ttacataaag agggcagcag caaaacaagc caaggcggcc aagagatcaa actctgtccg 721 ccaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcct 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctatct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taatgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga cataggatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgaacg gaaagatgag 2161 aataagttcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aagctcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcaactcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaagcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccctacaag agccatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat tagagaccag aggatcagat atattgagga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cggttccgca aggcacatgg tcgattgaaa tctcctgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acaatttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa aaccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccatcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagagaatt tggcagaact agaaagggac ttaaaggaga tcaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa atgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcgtatat cgatggttta gatttaaatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS L54 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L54. ACCESSION L54 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L54" /host="Trifolium pratense" /db_xref="taxon:12049" /country="Australia: Reddestone, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKILLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSRLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQEPSMALNGM TPVMTNSRYITRGMESPKSQAPSRSRSMS" BASE COUNT 1696 a 1199 c 1294 g 1420 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggtg gcagctcaag aagctgaaaa tctgtctttg atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttttgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg actatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcagtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgaccag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtt 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 attttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcacaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agccatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaatgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cggttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gtaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac cctgatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaaccg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttgagcg caaaagaaaa agaagaaatt 4561 tccagctcaa aaccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt gtatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagagaatt tggcagaact agaaagggat ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagatgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca agattaggag ggtctcttag aagtggagaa 4981 tcgtccctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS L61 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L61. ACCESSION L61 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L61" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Shannon Vale, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVINFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVAISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKDKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSANHSLMEYSRPTMNIRSRV SYYNSSPRPLPPRQAPSLMSLTHTANTRKFNRFLTNSASPRAVRNDSPQEPSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1699 a 1206 c 1290 g 1414 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgcgg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtctttg atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttaactttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttgc ataatgcatt ggtggcggta gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaggat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgttt acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ccaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaatcg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagttgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcacaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaaa 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 caattcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat tcaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agccatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cggttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgac 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtagccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca aggataagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa aaccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagagaatt tggcagaact tgaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tctaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS L70 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L70. ACCESSION L70 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L70" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLSSRMNYIKRAASKKAKAVKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKKGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPD ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLTGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGNPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1700 a 1203 c 1289 g 1417 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgtcct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggtc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa aaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ctggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcattact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc agcacatgca cagttggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtgtcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccaat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt tttttagatc 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccaaaact ccgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaacaac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccgacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataatttc 4201 tgcatatcat atggagcttg gacagaccgt gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca cgggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagtg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcac aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ccccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtaa tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagcc tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS L76 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L76. ACCESSION L76 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L76" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEQDVLVAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDKDSSSSSSSIVSSRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESDDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQEPSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1693 a 1200 c 1298 g 1418 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgatggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacag gacgtgctag tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtctttg atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcag aagaaggagg tactttccgc caccgagcaa atgattaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgttca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 ccatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccagtgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgct acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ctggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgttt acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggat 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagtaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcacaccaca gcgccgacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agccatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cggttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcagaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgata aagatagtag tagtagtagt agtagtatag ttagtagtcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa aaccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagagaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcagatga tccacctaag 4921 aaggcaggaa cactcaaggg gactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaggtgc 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tggcggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS L85 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L85. ACCESSION L85 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L85" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAVKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYLVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTISFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDAQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISNSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1706 a 1197 c 1286 g 1420 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtctttg atggacccag agccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggta aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccaga caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtcccagaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgaaaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcta aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgctca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtaatat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ccttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaatgca 3781 aactggacga acatcaaagc acaatggtat tcccaaaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga tccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaacaac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagttga gaaagatgga acaatttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agacagccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgc ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccaactcaa agccctctaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgttca catttctatt tgacggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS L86 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate L86. ACCESSION L86 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="L86" /host="Trifolium repens" /db_xref="taxon:12049" /country="Australia: Lambs Valley, NSW" /collection_date="2022" /collected_by="Joop van Leur" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHFGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHISVNRTHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVVPLVAAYGYPSETGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQSVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLNLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRTPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQEPSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1700 a 1203 c 1288 g 1418 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg catgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac agccatgcgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtctttg atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaac aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttatattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgattttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt caggcctgag cattttggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc ttaagaatga 1081 tattgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcacagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggactc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagtcg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag cggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatcg 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcactacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcacaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agccatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac cgacgccagc caaacatgag 3661 agatttattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cggttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccga gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgattggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatatta gcgtcaatcg cactcaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggcaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa aaccctcaaa tgttgaacgt caagttgttc ctcttgttgc agcctatgga 4621 tacccgtcgg aaacaggtgt ctacgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagagaatt tggcagaatt agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acctccgatt tggcgcaatg ccgaccagtc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta aatttagatt aatatagtag aagtaagtgc 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg ttaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtt 5581 cctttgataa cgttaaatga aagcggagt // LOCUS SS1 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate SS1. ACCESSION SS1 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="SS1" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: South Stirlings, WA" /collection_date="2020" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQRQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDISHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND VERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLQDSFTPVEYVSDEDSSSSGSSIVSNRPSTPDNDSDVQFAESLKGKLP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPMRVIQEDDPDYTPPIWRNA DQAVVIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMHARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1698 a 1206 c 1291 g 1414 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gctaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgagg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaagac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcaag aagctgaaaa cctgtcttta atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc tgactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattggagcc 841 tgaacccaaa ttaaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatattag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccagg caaattaggc aggagttggg 1141 cgttctaaac gccgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagttttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaaaaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaaccttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catcgaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcgca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaagga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacagataa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ttttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagctcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttt 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt agctggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat ttggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaggaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg catccaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtggt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagct tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccgatgag ggtgatccag 4801 gaagacgatc ctgattacac acccccgatc tggcgcaatg ccgaccaggc tgttgtgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcatgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacataggc gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS WA8 5609 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate WA8. ACCESSION WA8 VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5609) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5609 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="WA8" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Torbay, WA" /collection_date="2018" /collected_by="Benjamin Congdon" gene 80..2760 /gene="RdRp" CDS join(80..1162,1162..2760) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLRDGAPLKTWPQP CAPMGSFRSSDDFQVAAREAENLSSMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQMIKHAYEEHIGEAPFGKWFNTLPSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGELLEPEPKLKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND VERAKIVHSPAAKKIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKEGIFSN LDYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2841..2981 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2971..5139 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3571..3573,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQFMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLTGPAVKKTAKYNFCISYGAWTDRDMELGLVSVVLDEHLEGVRGSQYVKKTLRP GHVSVNRIHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKCP TQSKLPPKGFLSRLSAKEKEEISSSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPERVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDPPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQRILNNLGKMRARAYIDGLDLD" gene 2971..3570 /gene="CP" CDS 2971..3570 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQFMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2987..3553 /gene="MP" CDS 2987..3553 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSRPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1706 a 1203 c 1288 g 1412 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 ttaaatacta gttaatttaa tgtttaattt cgatagttta gtgtcggcca ccgctaaggt 121 ggtcaaagat tttattcatt tttgttataa tagggcgcgg cacgtttact atgctctcaa 181 acgctggctc tgggagctcc agggcgtgtt tgctgcacat gacgcttttg tcgacatgtg 241 ctacgacgct atgtatggcg tcgaagagtt tgagtgggaa ctgcaaaaac agttctcaag 301 tgccgaacac gacgtgctaa tcgccaagca cgaatttgag cgcctgttaa gggatggcgc 361 cccattaaag acatggccac aaccatgtgc tcctatgggc agtttccggt cgtctgatga 421 cttccaggta gcagctcgag aagctgaaaa cctgtcttca atggacccag aaccctcctt 481 gattaaaggg tctggtgatt actcgcttga caatcctaat aggattgaaa agttcatcaa 541 cctcgttcaa aagaaggagg tactttccgc caccgagcaa atgatcaaac atgcttatga 601 agagcatatc ggtgaggcac cattcggaaa gtggttcaac actctgccct ctcgcatgaa 661 ttacattaag agggctgctt caaagaaggc caaggcggcc aagagatcaa actctgtccg 721 tcaaatggta aacgaggtaa atgtcatccc agactttatc tcaatttgtg atgttgtcca 781 ggtggatact ggcgagaagc tccctcccaa gaaagataaa gacggggagc tattagagcc 841 tgaacccaaa ttgaaaatga tgagaagggt cagacctgag cattatggtg atgctcgttc 901 ttacataagg cagcacattc gcaacaataa catgcgcctt attgacggct ctgatgttag 961 tcatgctacc atcaaccgat atgctctcaa gttctgcgaa gacttagagc ttgatatgac 1021 cagtactgca atgctggttg attatgctat gacaatggtg cccatccctc tcaagaatga 1081 tgttgaaagg gcgaagattg tccattctcc tgcagccaaa aaaattaggc aggagttggg 1141 cgttctaaac gctgaggttt tttagagggg ctctgctccg actctggttt tgaatccccg 1201 ttttctattt tggggttgcc agagatcgtg gtgcgtagtg gagctacacc taggaagagt 1261 cgtagtgtaa ttagctttct ttcgcagttt actttaggtc tagattacca atgcccaaat 1321 cccagtttac ataatgcatt ggtggcggtt gaacgtaggg tgttcaccgt tgggaaagga 1381 aatgaggtag tgctacctta caagaacaaa gaaggaatat tttccaatct tgattacttt 1441 cgagactcaa tagtcaacaa agttggttgt ccaaggaccc acactcctga ggagcttgct 1501 gcaacgtacc actctggaaa gagaagtctg tacaatgcag ctgttcaaag cctcaagaag 1561 aaggcagttg aaaggagtga tgctaacgtg acagcctttc tcaagatgga aaaacatctg 1621 atgagtaaga agatagcacc caggttgata tgtccccgaa acaaaagata caatgttgaa 1681 ttgggacgtc gattgaaatt taacgagaag aaattcatgc atgcaatcga ctcaactttt 1741 gaatccccaa ctgttcttag tggatatgac agttttaaag ttgggaagat aatagccaat 1801 aaatggtcca aatttaagag accagttgca ataggtgttg atgcgagcag atttgatcag 1861 cacgtggggg tagaagcact ccaatgggag cactcgattt acaacggtgc attcaaagat 1921 cccattctca aggagttgtt acactggcaa acagagaata gaatcatgct gtttgttgaa 1981 gacaaaatcc tcaagttcaa ggtcaaagga catagaatgt ccggcgacat taacacctct 2041 tctggcaaca aattaatcat gtgtggtatg atgcactact acttcaagac acttggagtc 2101 aaagccgaac tctgcaacaa tggcgatgat tgtgttatca tatgtgagcg gaaagatgag 2161 aataaattcc aacacatgca cagctggttt aaagactatg ggtttgacat gcagatagag 2221 actcctgtct acaagattgg acagatagag ttttgtcaaa gtaaaccagt taaaattaat 2281 ggctattata gaatggtgcg taaaccagag agcatatcca aagacgccca ttccctcatt 2341 tccatggcat cagctgaaga tgtcaaaact ttcatgagtg ctaccgccca atgcggtatg 2401 atcttgaata gtggtgtacc tgttcttgat gcttatcata aatgcctatt taaagcatca 2461 gggtacaaga aagtatcaca agaagcaata gaaagaattg tctcttttgg aacgcaggac 2521 aaactcgggc tcaggaaaga gcgagtcgaa gaaccaataa caatggataa taggttgagt 2581 tattgggagt ccagtggggt tgaccctcag acacaggtcc ttgtcgaacg atattttgac 2641 aatctgacgg tccatatcga accccgcggc gtaaagagat tgacgccttt actagataaa 2701 actttgcttt caattgcaag tgtagcacga acttctgtgt cgctaccgat actttcaaaa 2761 tagaagcact catagtaacc atagtagtat tagtattaat agtagttagt ttattagatt 2821 acgctagctc gtacaggtaa attgcaacgg attacaaatt cctttcaggt tttgcttctg 2881 gattcatttc cagcattcct gtatcaatat tggcaattta ctttgtctac ctcaaaattt 2941 cgaaaaatct aagggagata ataaacgagt atggtcgcgg ttagcaatgt cgcattacaa 3001 cgaagacgca ctcgcagagc cgcaagacgc gctccacgag ttcagttcat ggctattcca 3061 acgaccagtc gcgcaccaca gcgcagacga agacaacgac gacgaaggag aaataatgga 3121 ggaggaggca atatggccgg aggatcaggc aaggctcaca aattccagtt ttcgaaggac 3181 ggtatcaatg gcagttccaa gggaactatc acattcgggc cgtctttatc agagtgcaag 3241 ccactctctg atggaatact caaggcctac catgaatata agatcacgag tatcttatta 3301 cagttcatct ccgaggcctc ttccacctcg tcaggctcca tcgcttatga gcttgaccca 3361 cactgcaaat acacggaaat ccaatcgctt cttaacaaat tcagcatcac caagagcggt 3421 tcgaaacgat tccccacaag agctatcaat ggccttgaat ggcatgacac cagtgatgac 3481 caattcaaga tacattacaa ggggaacgga aagtccgaaa tcgcaggctc cttcaagatc 3541 acgatcgatg tcttaactca gaatcccaaa taggtagacg gagaaccggg gccgaaacca 3601 ggtccggacc ccacaccccc accagcacca ccacctgaac caacgccagc caaacatgag 3661 agattcattg tgtacaccgg tacactgtca acgctcatta gtgcccgaca aaactcagat 3721 agtatatccc tctactcaat cagagaccag aggatcagat atattgaaga tgagaacgca 3781 aactggacga acatcaaagc acaatggtat tcccagaatt ctgtggaagc aataccaatg 3841 tttatttacc cagttccgca aggcacatgg tcgattgaaa tctcttgtga aggctaccag 3901 gcaacatcaa gcaccacgga cccccaccgg gggaaatctg atggaatgat tgcttatgat 3961 gacaatcaag ataaggtgtg gaatattggt gtgcaaaata atgtaaccat atccaataac 4021 aaggccgatg ctgactggaa atacggtcac ccagatttaa ctatcaacgg tgaacgtttc 4081 gaccaaaagc aagtagtcga gaaagatgga acagtttcgt ttcatttagt aactactggc 4141 cccaacgcga gtttcttttt aactggccct gccgttaaga agactgccaa gtataacttc 4201 tgcatatcat atggagcttg gacagaccgc gatatggaat taggattggt atcagttgtt 4261 ctcgatgaac atttagaggg tgttagaggt tctcagtacg ttaagaaaac gctaaggcca 4321 ggtcatgtta gcgtcaatcg catccaccga ttgcaagaca gtttcacacc ggtggaatat 4381 gtcagtgatg aagatagtag tagtagtagt agtagtatag ttagtaatcg accatcaact 4441 ccggataatg atagtgatgt ccaatttgca gaatcgctca agggtaagtg tccgacgcag 4501 tcgaagcttc cacctaaagg attcctatca cggttaagcg caaaagaaaa agaagaaatt 4561 tccagctcaa agccctcaaa tgttgaacgt caagttggtc ctcttgttgc agcctatgga 4621 tacccgtcgc aaacaggtgt ctatgatgca gccagagaga tcctgcaagc taaagaagct 4681 gcagaaaatt tggcagaact agaaagggac ttaaaggaga ttaacaagct agaaccgccg 4741 gatacaatag ttcaggaaga aatccctgac ttcgtgccac ctccggagag ggtgatccag 4801 gaagacgatc ctgattacat acccccgatt tggcgcaatg ccgaccaggc tgttacgatc 4861 gccacaagtg gtcaaataga ctggagtcgg ccagcttatg agtcaggtga tccacctaag 4921 aaggcaggaa cactcaaggg aactctctca aaattaggag ggtctcttag aagtggagaa 4981 tcgtctctta gaggtaacct ccgcaagacg caagatcaaa ctgatctaga caacaaattg 5041 tccaaactta gtgttattca aagatctcaa tatcagagaa ttctaaataa cctagggaaa 5101 atgcgtgcgc gagcatatat cgatggttta gatttagatt aatatagtag aagtaagtgt 5161 gcgctttcca tcacatagac gtggagccaa ccttggtgag ttgctgtcgt gggatcctgg 5221 gaaacaggtt cggtgaacaa aaagccggta atctcgggtg ttcgtcaaac accgtcgtat 5281 caacacagta tgcatatcca tcaataaccc actgtccttg tgactaaaaa ttagagttag 5341 tttaccgcct ctcttgtaaa ccacatacaa gatgcgagct tgtggagagt accatccctt 5401 gaataaggat ggttgaaagt ttctggttcc aatccattga tcaaaattgg cactgcttct 5461 ggtgactaca ctgccgctgg tgatacagcg taaacaattt ctgctactta ctcgatgaaa 5521 tgagagtaac tagtaaagtc ttgccgctca catttctatt tgacggtact aggcttcgtc 5581 cctttgatga cgttaaatga aagcggagt // LOCUS McG 5611 bp RNA linear VRL 17-JUN-2024 DEFINITION Soybean dwarf virus isolate McG. ACCESSION McG VERSION KEYWORDS . SOURCE Soybean dwarf virus ORGANISM Soybean dwarf virus Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes; Tolivirales; Tombusviridae; Regressovirinae; Luteovirus; Luteovirus glycinis. REFERENCE 1 (bases 1 to 5611) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Genetic diversity of soybean dwarf virus in two mixed-cropping regions of Australia JOURNAL unpublished REFERENCE 2 (bases 1 to 5611) AUTHORS Kehoe,M.A., Congdon,B.S. and Sharman,M. TITLE Direct Submission JOURNAL Submitted (17-JUN-2024) DPIRD Diagnostic Laboratory Service, Department of Primary Industries and Regional Development, 3 Baron-Hay Court, South Perth, WA 6151, Australia COMMENT Bankit Comment: ALT EMAIL:monica.kehoe@gmail.com Bankit Comment: TOTAL # OF SEQS:41 ##Assembly-Data-START## Assembly Method :: CLC Genomics Workbench v. 2022; Geneious Prime v. Various Sequencing Technology :: Illumina ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..5611 /organism="Soybean dwarf virus" /mol_type="genomic RNA" /isolate="McG" /host="Trifolium subterraneum" /db_xref="taxon:12049" /country="Australia: Busselton, WA" /collection_date="2020" /collected_by="Benjamin Congdon" gene 82..2762 /gene="RdRp" CDS join(82..1164,1164..2762) /gene="RdRp" /ribosomal_slippage /note="P1-P2 frameshift fusion protein" /codon_start=1 /product="RNA-dependent RNA polymerase" /translation="MFNFDSLVSATAKVVKDFIHFCYNRARHVYYALKRWLWELQGVF AAHDAFVDMCYDAMYGVEEFEWELQKQFSSAEHDVLIAKHEFERLLKDGAPLRTWPQP CAPMGSFRSSDDFQVAAQEAENLSLMDPEPSLIKGSGDYSLDNPNRIEKFINLVQKKE VLSATEQLIKHAYEEHIGEAPFGKWFNTLSSRMNYIKRAASKKAKAAKRSNSVRQMVN EVNVIPDFISICDVVQVDTGEKLPPKKDKDGEPLEPEPKMKMMRRVRPEHYGDARSYI RQHIRNNNMRLIDGSDVSHATINRYALKFCEDLELDMTSTAMLVDYAMTMVPIPLKND IERAKIVHSPAARQIRQELGVLNAEVFLEGLCSDSGFESPFSILGLPEIVVRSGATPR KSRSVISFLSQFTLGLDYQCPNPSLHNALVAVERRVFTVGKGNEVVLPYKNKPGIFSN LNYFRDSIVNKVGCPRTHTPEELAATYHSGKRSLYNAAVQSLKKKAVERSDANVTAFL KMEKHLMSKKIAPRLICPRNKRYNVELGRRLKFNEKKFMHAIDSTFESPTVLSGYDSF KVGKIIANKWSKFKRPVAIGVDASRFDQHVGVEALQWEHSIYNGAFKDPILKELLHWQ TENRIMLFVEDKILKFKVKGHRMSGDINTSSGNKLIMCGMMHYYFKTLGVKAELCNNG DDCVIICERKDENKFQHMHSWFKDYGFDMQIETPVYKIGQIEFCQSKPVKINGYYRMV RKPESISKDAHSLISMASAEDVKTFMSATAQCGMILNSGVPVLDAYHKCLFKASGYKK VSQEAIERIVSFGTQDKLGLRKERVEEPITMDNRLSYWESSGVDPQTQVLVERYFDNL TVHIEPRGVKRLTPLLDKTLLSIASVARTSVSLPILSK" CDS 2843..2983 /note="ORF3a; P3a expressed by leaky scanning of subgenomic mRNA" /codon_start=1 /product="P3a" /translation="- ATDYKFLSGFASGFISSIPVSILAIYFVYLKISKNLREIINEYGRG" CDS 2973..5141 /note="readthrough domain protein" /codon_start=1 /transl_except=(pos:3573..3575,aa:OTHER) /product="P3/P5" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPKXVDGEPGPKPGPDPTPPP APPPEPTPAKHERFIVYTGTLSTLISARQNSDSISLYSIRDQRIRYIEDENANWTNIK AQWYSQNSVEAIPMFIYPVPQGTWSIEISCEGYQATSSTTDPHRGKSDGMIAYDDNQD KVWNIGVQNNVTISNNKADADWKYGHPDLTINGERFDQKQVVEKDGTVSFHLVTTGPN ASFFLAGPAVKKTAKYNFCISYGAWTDRDMEFGLVSVVLDEHLEGVRGSQYVRKTLRP GHVSVNRIHRLQDSFTPVEYVSDEDSSSSSSSIVSNRPSTPDNDSDVQFAESLKGKYP TQSKLPPKGFLSRLSAKEKEEISNSKPSNVERQVGPLVAAYGYPSQTGVYDAAREILQ AKEAAENLAELERDLKEINKLEPPDTIVQEEIPDFVPPPARVIQEDDPDYIPPIWRNA DQAVTIATSGQIDWSRPAYESGDQPKKAGTLKGTLSKLGGSLRSGESSLRGNLRKTQD QTDLDNKLSKLSVIQRSQYQGILNNLGKMRARAYIDGLDLD" gene 2973..3573 /gene="CP" CDS 2973..3573 /gene="CP" /note="Coat Protein" /codon_start=1 /product="ORF3" /translation="MVAVSNVALQRRRTRRAARRAPRVQLMAIPTTSRAPQRRRRQRR RRRNNGGGGNMAGGSGKAHKFQFSKDGINGSSKGTITFGPSLSECKPLSDGILKAYHE YKITSILLQFISEASSTSSGSIAYELDPHCKYTEIQSLLNKFSITKSGSKRFPTRAIN GLEWHDTSDDQFKIHYKGNGKSEIAGSFKITIDVLTQNPK" gene 2989..3555 /gene="MP" CDS 2989..3555 /gene="MP" /note="Movement Protein" /codon_start=1 /product="ORF4" /translation="MSHYNEDALAEPQDALHEFSSWLFQRPVAHHSADEDNDDEGEIM EEEAIWPEDQARLTNSSFRRTVSMAVPRELSHSGRLYQSASHSLMEYSKPTMNIRSRV SYYSSSPRPLPPRQAPSLMSLTHTANTRKSNRFLTNSASPRAVRNDSPQELSMALNGM TPVMTNSRYITRGTESPKSQAPSRSRSMS" BASE COUNT 1709 a 1206 c 1282 g 1414 t ORIGIN 1 acaagatcca cctcccggca ccttcgtatc gtgtttgagg tatctctagt gtttggttgt 61 tttaaatact agttaatttt gatgtttaat ttcgatagtt tagtgtcggc caccgctaag 121 gtggtcaaag attttataca tttttgttat aatagggcga ggcatgtata ttacgctctc 181 aaacgctggc tctgggaact ccagggcgtg tttgctgcgc atgacgcttt tgtcgacatg 241 tgctacgacg ccatgtatgg cgttgaagag tttgaatggg aattgcaaaa acaattctca 301 agtgctgaac atgacgtgct tatcgccaag cacgaatttg agcgtctatt aaaagatggc 361 gccccattaa ggacatggcc acaaccatgc gctcctatgg gcagtttccg gtcgtctgac 421 gacttccagg tggcagccca agaagctgaa aacctgtccc tgatggaccc agaaccctcc 481 ttgattaaag ggtctggtga ttactcgctt gacaatccta acaggattga aaagttcatt 541 aacctcgttc aaaagaagga ggtactttcc gccaccgagc aattgatcaa acatgcttat 601 gaagagcata tcggtgaggc accattcgga aagtggttca acaccctgtc ctctcgtatg 661 aactatataa agagggctgc ttcaaagaag gccaaggcgg ctaagagatc aaactctgtc 721 cgtcaaatgg taaacgaggt aaatgtcatt cctgacttta tctcaatatg tgatgttgtc 781 caggtggata ctggcgagaa actccccccc aagaaagaca aagacgggga gccactagag 841 cctgaaccca aaatgaaaat gatgagaagg gttaggcccg agcattatgg tgatgctcgt 901 tcttacatta ggcagcatat tcgcaacaac aacatgcgcc taattgacgg ctctgatgtt 961 agccatgcta ccatcaaccg atatgctctt aagttttgcg aagacttaga gcttgatatg 1021 accagtactg caatgctggt tgattatgct atgacaatgg tgcccatacc cctcaaaaat 1081 gacattgaaa gggcgaagat tgtccattct cctgcagcca ggcaaattag gcaggagttg 1141 ggcgttctaa acgctgaggt tttttagagg ggctctgctc cgactctggt tttgaatccc 1201 ctttttctat tttgggatta ccagagatcg tggtgcgtag tggagctaca cctaggaaga 1261 gtcgtagtgt aattagtttt ttatcgcagt ttactttagg tctagattac caatgcccca 1321 atcccagttt acacaatgca ttggtggcgg ttgaacgtag agtgttcacc gttgggaaag 1381 gaaatgaggt agtgctacct tacaagaaca aaccaggaat attttccaat cttaattact 1441 ttcgcgactc aattgtcaac aaagttggtt gtccaaggac ccacactcct gaggaactag 1501 ccgcaacgta ccactctgga aagagaagtc tgtacaatgc tgcagttcaa agccttaaaa 1561 agaaggcggt cgaaaggagt gatgctaacg tgacggcctt tcttaagatg gagaaacacc 1621 ttatgagtaa gaagatagca cccaggttga tatgtcctcg aaacaaaaga tacaatgttg 1681 aattgggacg tcgattgaaa tttaacgaga agaaattcat gcatgcaatc gactcaacct 1741 ttgaatcccc aactgttctt agtggatatg acagttttaa agttgggaag ataatagcca 1801 ataaatggtc caaatttaag agaccagttg caataggtgt tgatgcgagc agatttgatc 1861 agcacgtggg ggtagaagca ctccaatggg agcactcgat ttacaacggt gcatttaaag 1921 atcccattct caaggagttg ttacactggc aaacagagaa cagaatcatg ctgtttgttg 1981 aagacaaaat cctcaagttc aaggtcaaag gacatagaat gtccggcgat attaacacct 2041 cttctggcaa caaattaatc atgtgtggta tgatgcacta ctacttcaag acacttggag 2101 tcaaagccga actctgcaac aatggcgatg attgtgttat catatgtgag cggaaagatg 2161 agaataaatt ccaacacatg cacagctggt ttaaagacta tgggtttgac atgcagatag 2221 agactcctgt ctacaagatt ggacagatag agttttgtca aagtaaacca gttaaaatta 2281 atggctatta tagaatggtg cgtaaaccag agagcatatc caaagacgcc cattccctca 2341 tttccatggc atcagctgaa gatgtcaaaa ctttcatgag tgctaccgcc caatgcggta 2401 tgatcttgaa tagtggtgta cctgttcttg atgcttatca taaatgccta tttaaagcat 2461 cagggtacaa gaaagtatcg caagaagcaa tagaaagaat tgtctctttt ggaacgcagg 2521 acaaactcgg gctcaggaaa gagcgagtcg aagaaccaat aacaatggat aataggttga 2581 gttattggga gtccagtggg gttgaccctc agacacaggt ccttgtcgaa cgatattttg 2641 acaatctgac ggtccatatc gaaccccgcg gcgtaaagag attgacgcct ttactagata 2701 aaactttgct ttcaattgca agtgtagcac gaacttctgt gtcgctaccg atactttcaa 2761 aatagaagca ctcatagtaa ccatagtagt attagtatta atagtagtta gtttattaga 2821 ttacgctagc tcgtacagat aaattgcaac ggattacaaa ttcctttcag gttttgcttc 2881 tggattcatt tccagcattc ctgtatcaat attggcaatt tactttgtct acctcaaaat 2941 ttcgaaaaat ctaagggaga taataaacga gtatggtcgc ggttagcaat gtcgcattac 3001 aacgaagacg cactcgcaga gccgcaagac gcgctccacg agttcagctc atggctattc 3061 caacgaccag tcgcgcacca cagcgcagac gaagacaacg acgacgaagg agaaataatg 3121 gaggaggagg caatatggcc ggaggatcag gcaaggctca caaattccag ttttcgaagg 3181 acggtatcaa tggcagttcc aagggaacta tcacattcgg gccgtcttta tcagagtgca 3241 agccactctc tgatggaata ctcaaagcct accatgaata taagatcacg agtatcttat 3301 tacagttcat ctccgaggcc tcttccacct cgtcaggctc catcgcttat gagcttgacc 3361 cacactgcaa atacacggaa atccaatcgc ttcttaacaa attcagcatc accaagagcg 3421 gttcgaaacg attccccaca agagctatca atggccttga atggcatgac accagtgatg 3481 accaattcaa gatacattac aaggggaacg gaaagtccga aatcgcaggc tccttcaaga 3541 tcacgatcga tgtcttaact cagaatccca aataggtaga cggagaaccg gggccgaaac 3601 caggtccgga ccccacaccc ccaccagcac caccacctga accaacgcca gccaaacatg 3661 agagattcat tgtgtacacc ggtacactgt caacgctcat tagtgcccga caaaactcag 3721 atagtatatc cctctactca atcagagacc agaggatcag atatattgaa gatgagaacg 3781 caaactggac gaacatcaaa gcacaatggt attcccagaa ttctgtggaa gcaataccaa 3841 tgtttattta cccagttccg caaggcacat ggtcgattga aatctcttgt gaaggctacc 3901 aggcaacatc aagcaccacg gacccccacc gggggaaatc tgatggaatg attgcttatg 3961 atgacaatca agataaggtg tggaatattg gtgtgcaaaa taatgtaacc atatccaata 4021 acaaggccga tgctgactgg aaatacggtc acccagattt aactatcaac ggtgaacgtt 4081 tcgaccaaaa gcaagtagtc gagaaagatg gaacagtttc gtttcattta gtaactactg 4141 gccccaacgc gagtttcttt ttagctggcc ctgccgttaa gaagactgcc aagtataact 4201 tctgcatatc atatggagct tggacagacc gcgatatgga atttggattg gtatcagttg 4261 ttctcgatga acatttagag ggtgttagag gttctcagta cgttaggaaa acgctaaggc 4321 caggtcatgt tagcgtcaat cgcattcacc gattgcaaga cagtttcaca ccggtggaat 4381 atgtcagtga tgaagatagt agtagtagta gtagtagtat agttagtaat cgaccatcaa 4441 ctccggataa tgatagtgat gtccaatttg cagaatcgct caagggtaag tatccgacgc 4501 agtcgaagct tccacctaaa ggattcctat cacggttaag cgcaaaagaa aaagaagaaa 4561 tttccaactc aaagccctca aatgttgaac gtcaagttgg tcctcttgtt gcagcctatg 4621 gatacccgtc gcaaacaggt gtctatgatg cagccagaga gatcctgcaa gctaaagaag 4681 ctgcagaaaa tttggcagaa ctagaaaggg acttaaagga gattaacaag ctagaaccgc 4741 cggatacaat agttcaggaa gaaatccctg acttcgtgcc acctccggcg agggtgatcc 4801 aggaagacga tcctgattac atacccccga tttggcgcaa tgccgaccag gctgttacga 4861 tcgccacaag tggtcaaata gactggagtc ggccagctta tgagtcaggt gatcaaccta 4921 agaaggcagg aacactcaag ggaactctct caaaattagg agggtctctt agaagtggag 4981 aatcgtctct tagaggtaac ctccgcaaga cgcaagatca aactgatcta gacaacaaat 5041 tgtccaaact tagtgttatt caaagatctc aatatcaggg aattctaaat aacctaggga 5101 aaatgcgtgc gcgagcatat atcgatggtt tagatttaga ttaatatagt agaagtaagt 5161 gtgcgctttc catcacatgg gcgtggagcc aaccttggtg agttgctgtc gtgggatcct 5221 gggaaacagg ttcggtgaac aaaaagccgg taatctcggg tgttcgtcaa acaccgtcgt 5281 atcaacacag tatgcatatc catcaataac ccactgtcct tgtgactaaa aattagagtt 5341 agtttaccgc ctctcttgta aaccacatac aagatgcgag cttgtggaga gtaccatccc 5401 ttgaataagg atggttgaaa gtttctggtt ccaatccatt gatcaaaatt ggcactgctt 5461 ctggtgacta cactgccgct ggtgatacag cgtaaacaat ttctgctact tactcgatga 5521 aatgagagta actagtaaag tcttgccgct cacatttcta tttgacggta ctaggcttcg 5581 tccctttgat gacgttaaat gaaagcggag t //